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The Spanish guitar's tuning mixes four perfect fourths (five semitones) and one major-third, the latter occurring between the G and B strings: E-A-D-G-B-E.
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Auto-calibration was performed daily using Agilent tuning mix HP0321 (Agilent Technologies) prepared in acetonitrile.
ESI tuning mix (Agilent, Palo Alto, CA) was used as a calibration standard.
ES tuning mix (Agilent Technologies, Palo Alto, CA, USA; reorder no. G2421A) was used for external mass calibration.
Mass spectra were calibrated externally with respect to the ions of an ES Tuning Mix (Agilent Technologies).
A Tuning Mix mass spectrometry standard (Tunemix, G2421A, Agilent Technologies, Santa Clara, CA) was used as a mobility calibration standard.
Mass calibration was done with a commercial mixture of perfluorinated trialkyltriazines (ES Tuning Mix, Agilent Technologies, Santa Clara, CA, USA).
A Tuning Mix calibration standard (TuneMix, G24221A) was purchased from Agilent Technologies (Santa Clara, CA) and used as received.
Source and transfer parameters were optimized by direct infusion of an ESI tuning mix (Agilent Technologies, Waldbronn, Germany).
Agilent ESI-L Low Concentration Tuning Mix was spotted nearby on the NIMS chip to allow for mass calibration of the instrument.
The m/z scale of all mass spectra was externally calibrated using G2421A electrospray "tuning mix" from Agilent Technologies (Santa Rosa, CA, USA) as calibration standard.
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