Following a tuning protocol that was validated on a random selection of slides, positively stained pixels were digitally selected on all slides with the following parameters in ImageScope software (v 11.2, positive pixel count algorithm v 9.1): view width/height 1000, zoom 1, hue value 0.1, hue width 0.5, and color saturation threshold 0.04.
Drift speed, window size and timing of stimulus presentation were the same as for the orientation tuning protocol.
The interview questions were piloted to fine-tune the protocol prior to the study interviews.
The protocol can be tuned by setting the protocol parameters.
Based on the RNAscope ISH principle, we provide a fine-tuned protocol that we developed for large tissue samples, as exemplified here by using whole-mount zebrafish embryos.
As shown in Figure 3, employing the fine-tuned protocol, cxcl12a, an RNA encoding for a chemoattractant for PGCs [ 22], is specifically detected at a high resolution in 12-hpf embryos.
Sophisticated routing protocols, tuned to various specific needs, can be layered on top of FCP.
On vehicle-to-roadside communication, in order to retain compatibility with the original IMS-AKA protocol, we have fine-tuned the original protocol.
When used in combination with protocols specifically tuned to a particular media format, called media-specific protocols, cyclic-UDP can amortize the degradation in output quality caused by burst packet losses over a long period.
The animal species to which this protocol was tuned lies at the approximate median of 18 species of deposit-feeder dissolution capability for PAH [39].
Previous works in [18] and [19] have shown that if exact knowledge (e.g., number of active stations and packet size) of the network is known, the CW size can be tuned to achieve a protocol capacity very close to its theoretical bound.
