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"About 24 hours later, the tumour area goes black, a couple of days later you see a scab, and at around the 1.5 week mark, the scab falls off, leaving clean skin with no tumour there.
Tumour area and volume were calculated as previously described.
The degree of necrosis was recorded at low magnification (× 40) and scored as 0, absent; 1, focal (⩽10% of the tumour area); 2, moderate (10%30%% of the tumour area); 3, extensive (⩾30% of the tumour area).
Area of necrosis was expressed as the percentage of total tumour area.
The artery (or arteries) supplying the tumour area was selectively catheterised.
In some instances, the tumour area showed less uptake than normal liver.
Fifty cells were counted in 10 random fields within the tumour area.
Care was taken that the hit-points covered the entire tumour area.
The amount of tumour necrosis was described in percentage of the evaluated tumour area.
By setting a threshold on the cytokeratin staining, a mask of the tumour area was created.
SPECT imaging using 99mTc-HYNIC-VEGF showed highly heterogeneous focal accumulation in the tumour area.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com