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Tumor dimensions were determined by averaging three repeated measurements.

Tumor dimensions were monitored using calipers at right angles every 3 to 4 days.

These techniques produce high-resolution images of the iris tumors resulting in excellent visualization of the tumor anatomy and measurement of tumor dimensions.

Tumor dimensions were measured with vernier calipers, and the volumes were calculated as follows: Tumor volume (mm3) = Width2 × Length / 2. When the tumors reached 100 mm3 (designated as day 1), the animals were randomized into the following treatment groups: free Sal (4 mg/kg), free Sal (8 mg/kg), and Sal NPs prepared by the single emulsion method (SE-NPs) (8 mg/mL).

Tumor dimensions were measured twice-weekly using calipers.

When tumors became measurable, tumor dimensions were estimated using slide caliber and the volume was calculated according the previously reported formula (L x W x H /2 [46].

Tumor dimensions were measured using a digital caliper two times a week and volumes estimated as previously described (volume = (width2×length)/2) [20].

Tumor dimensions were measured every other day and their volumes were calculated from caliper measurements of two orthogonal diameters (x and y, larger and smaller diameters, respectively) by using the formula volume = xy2/2.

Briefly, tumors were microdissected and tumor dimensions were measured with a ruler, and tumor volume was calculated using the formula [volume = width2×length×0.52] to approximate the volume of spheroid.

To gain a more quantitative measurement of tumor regression, the tumor dimensions were obtained manually by the use of calipers while the measurement of the bioluminescent region of interest (red circles) was obtained through the Living Image software from the ventral and lateral views.

The tumor dimensions

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