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In this regard it is interesting to note that in the -12°C growth experiments, none of the tubes froze after the first week of growth following inoculation suggesting that a product, possibly EPS, produced by the bacterium may have lowered the freezing point of the growth medium.
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Separated plasma was immediately removed and stored in capped 1.5-ml polypropylene tubes frozen at −80 °C until later analysis.
The sample was collected from random sites using a hand trowel and pooled into sterile tubes, frozen in dry ice and transported to the laboratory for further analysis.
A key part of this grand ambition lies in the lines of test tubes frozen in liquid nitrogen in a Californian zoo.
Urine samples were collected in 50-mL acid-washed tubes, frozen at −20°C, and shipped on dry ice.
Tissues were immediately placed separately in Eppendorf tubes, frozen on dry ice and stored at −80°C prior to analysis.
The bone samples were immediately put in sealed polyethylene test tubes, frozen and stored at -20°C until analysis.
CSF was collected, mixed by inversion and aliquoted in polypropylene tubes, frozen, and maintained –70 °C until analyzed.
Transferred suspension to fresh tubes, frozen with liquid nitrogen and sonicated in an ice-cold ultrasonic bath for 30 min.
The serum was then transferred by autoclaved pipettes to 4-ml endotoxin-free glass tubes, frozen and stored at -20°C.
Spot urine samples were collected in 50-mL acid-washed tubes, frozen at −20°C, and also shipped on dry ice.
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