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Seated on a high stool one morning last week, with tweezers in her left hand and small scissors in her right, she carefully clipped the kidney, liver and spleen from a small dead mouse splayed open before her, and put pieces in small test tubes for analysis.
After filtration through a glass wool filter, the samples were placed in NMR tubes for analysis.
At fixed times, after the containers were vortexed for 1 min, aliquots of 1 ml solution from each container were transferred into polypropylene test tubes for analysis.
Water samples for Fe and Mn species analysis were collected directly from hose to the plastic tubes for analysis both for total concentration (unfiltered samples) and for dissolved forms (filtered using a syringes Millipore 0.4 μm filters).
Twenty-four hours after transfusion, rats were sedated with ketamin and medetomidin as described and bled via the inferior caval vein in citrated (0.109 M) vacutainer tubes for analysis and blood culture.
Finally, the cells were washed twice, resuspended in staining buffer, and transferred to FACS tubes for analysis.
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Dissolved extracts were transferred to a 5-mm NMR tube for analysis.
Soils were ground to a uniform particle size with mortar and pestle and then loaded into a polyimide tube for analysis.
The supernatant was transferred to a new tube for analysis.
This extract was filtered through a 0.45 µm polyfluorotetraethylene syringe filter prior to transfer into an NMR tube for analysis.
We retained this first bronchoalveolar lavage fluid (BALF) in a separate tube for analysis of biochemistry content (LDH and protein).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com