Sentence examples for tube template from inspiring English sources

The method is based on replication of overhanging nanostructures from an aluminum tube template to polydimethylsiloxane (PDMS) via atomic layer deposition (ALD) assisted sacrificial etching.

Previously reported single-tube template preparation protocols, in fact, have been aimed at measuring only specific RNAs and employ lysis buffers containing low concentrations of the mild detergent NP-40 [ 25, 37], or they bypass the lysis step altogether and are limited to neuron studies [ 38].

The 3 E. coli like colonies selected from each stool were pooled into a common sample tube and template DNA was prepared from the pooled colonies.

For PCR amplification, the following components were combined in a 0.2-ml tube: cDNA template, 1× reaction buffer (Thermo Scientific), 2.0 mM MgCl2 (Thermo Scientific), 0.2 mM dNTPs (Qiagen), 0.8 M betaine (Sigma), 0.8 U Taq polymerase (Red Hot Taq from Integrated Sciences) and 10 pmol of each primer (forward and reverse primers as listed in Table 1).

Reaction tubes without template cDNA served as negative controls.

Mineralization appears to then progress within the organic walls of Alvinella spp. tubes, where fine iron sulphide (plus or minus a zinc and/or copper content) cores template tube layer and sublayer surfaces (Figs 3D F and 6C,D).

3. Add 10 μL DEPC H2O to each tube of the template, vortex briefly and centrifuge.

A sample was taken from each dilution tube as a template for the three PCR-based methods.

A sample (5 μl) was taken from each dilution tube as a template for the three PCR-based methods.

An additional control tube containing no template was included for each specific reaction using different primer sets.

Pairs of filter papers with smeared nasal swabs and with blood samples from the same animal were used in PCR tube directly as template RNA or kept at −70°C for viral stability study.