LDL was collected from the top fraction by tube slicing.
Finally, the tube was frozen in liquid nitrogen, the bottom of the tube sliced off and the cell-phage pellet transferred to a tube containing 50 ml culture of E. coli XL1-Blue in log phase and incubated for 15 min at room temperature to phage recovery.
For the preparation of HDL, the bottom fraction was then adjusted to d 1.21 g/ml with KBr, layered under KBr (d 1.21 g/ml) and centrifuged at 175,000× g/ml at 4 °C for 18 h and the top fraction containing HDL was collected by tube slicing.
The parameters of the helical multidetector CT scan were 120 kV, 80 mA, 0.8 s per tube rotation, slice thickness 3.75 mm, pitch 1.675 : 1 and table speed 33.5 mm per rot.
After wriggling on your back over a precipice, you shoot down a nearly vertical 60-foot-long slide, seemingly in free fall, before abruptly going horizontal into an acrylic tube that slices through a shark-infested tank.
While I roll collards into cigar-shaped tubes and slice them, he blends fistfuls of frozen spinach, blueberries, barley and supergreens — with a splash of flax oil, a heaping tablespoon of barley.
They used a power saw to trim away the aluminum skin of the tubes, then sliced the still-wet peat with a spatula.
Tubes were sliced, and two fractions were collected: non-HDL (VLDL+LDL), d<1.063 g/ml; and HDL, d>1.063 g/ml.
A separate dataset for each tube with a slice thickness of 5 mm and a reconstruction increment of 4 mm using a soft tissue kernel (B30f) was calculated.
CT acquisition parameters (e.g. tube current, voltage, slice thickness, rotation time and pitch) should be chosen with regard to the objective of the CT examination (e.g. attenuation and scatter correction, colocalisation of radiologically equivalent interpretation).
