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Culturing was performed at 35°C in liquid Mycobacteria growth indicator tube medium enriched with polymyxin B amphotericin B nalidixic acid trimethoprim azlocillin mixture and oleic acid albumin dextrose complex or on solid Löwenstein Jensen medium (Becton Dickinson, Alphen a/d Rijn, The Netherlands).
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The procedure was repeated several times between anaerobic liquid glucose medium without wheat straw and agar roll-tubes medium until the fungal colonies on the roll-tubes appeared uniform under the microscope.
Corresponding ball and bond type for high, tube for medium and wireframe for low layers.
Moreover a spiral with a long thin-walled silicone tube for medium transport is mounted inside the module.
Aliquots of all biliary samples were placed in a sterile glass tube containing medium for anaerobic and aerobic bacterial cultures.
After five days of culture, PBS was decanted into a special glass tube containing medium for fungi (Sabouraud 2 agar (SAB2-T), BioMerieux, Marcy l'Etoile, France).
The fresh pollen was cultured in Petri dishes in the liquid pollen germination and tube growth medium described by Mesejo et al. [ 48].
Co-labelling with FM4-64 was performed as described before [ 13, 27, 30] with 2 μM of the FM solution (Molecular Probes) prepared in pollen tube growth medium and observed 5-10 min after labelling.
Pipet tips (10), vacuum containers (10), plastic 15-mL tubes (10), syringes (10), glass slides (20), and tubes containing medium (10) were submitted for direct examination and culture for ameba to ensure that they were not contaminated.
The Special Steel division offers products such as bearing steel, gear steel, spring steel, seamless steel tubes and medium to thick plates.
The described one-dimensional simulator has been subjected to a wide range of numerical conditions which are constructed to represent currently simulated straight-tube porous medium experiments.
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