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The first tube content was acidified with concentrated HCl, whereas, the other was alkalinized with concentrated ammonia solution.
Boilers are classified according to their use, pressure, materials of construction, tube content, firing mode, and heat source.
SWNT film with high metallic tube content is found to have relatively good chemical stability against post treatments, as demonstrated from its stable film performance in ambient after annealing, and merely 15% reduction in sheet resistance upon sulfuric acid treatment.
The anterior midguts were then transferred to 100 µL of ice-cold MET solution (300 mM mannitol, 5 mM EGTA, 17 mM TRIS-base/HCl, pH 7.5) in a microcentrifuge tube and manually homogenized with an abrasive glass microhomogenizer during 5 min. 100 µL of ice-cold 24 mM MgCl2 were added to this preparation and the tube content was mixed.
The tube content was ultrasonicated for 3 min and then stirred for 10 min.
The image intensity of the tube content served as a reference to correct for gastric secretion.
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On the other hand, syringe aspiration may be inappropriate whenever CSF pressure needs to be measured with manometer tubing which then allows collection of tube contents via gravity.
Tube contents were removed via a pipette and rinsed with a CHCl3:MeOH (2 1) mixture.
Sample tubes were inverted several times to mix the tube contents, and aliquots were frozen at − 80 °C.
Tube contents were then boiled at 95°C for one hour.
The tube contents were incubated for 60 minutes at room temperature.
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