The high level of total suspended particles (TSP) contain large amount of organic and inorganic micropollutants (PAHs, Pb, Pt, Rh, etc).
Total soluble protein samples (TSP) containing 1 ng of recombinant hG-CSF (determined by immunoblot, data not shown) extracted from USH leaves and seeds were used for bioactivity assay.
In this study, we used the Test for Substitution Patterns, since the TSP contains the SLS and DLS and has been evaluated for assessment of altered movement patterns in the entire kinetic chain in individuals with ACL injury and in controls [ 3, 9].
All known TSPs contain at least one EGF domain with a consensus sequence for beta-hydroxylation of an asparagine residue, indicative of a capacity for calcium-binding, [ 43], and this trait was conserved in all the newly-identified fish TSPs (data not shown).
All the fish-encoded sequences identified as homologous to mammalian TSP-1 and TSP-2 contained vWF_C and TSP type 1 domains and were thus confirmed as belonging to TSP subgroup A. Those identified as subgroup B homologues on the basis of the oligomerization domain lacked these domains and included an additional EGF domain.
All the predicted fish TSP-3s and many of the TSP-4s contained a TSP amino-terminal domain.
TSPs consists of five subtypes encoded by independent genes, in which TSP-1 and TSP-2 contain type-1 repeated with anti-angiogenic activity [ 2, 3].
N-terminal amino acid sequence analysis confirmed that the recombinant protein included amino acid 18 550 of human TSP-2 containing the heparin-binding-domain, the procollagen-like domain, and the three type 1 (properdin-like) repeats.
Among them, TSP-1 and TSP-2 contain three identical subunits [ 9- 12], whereas TSP-3, TSP-4, and COMP (also called TSP-5) contain five identical subunits [ 8, 13, 14].
TSP 19 contains domains similar to taurine E, a sulfite exporter (eg, GenBank identifier CO741254.1).
