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Preliminary data show that the TS-3′ UTR polymorphism may be correlated with differential TS gene expression, with 6-bp deletions leading to lower intratumoral TS mRNA (Lenz et al, 2002a).
Neither TS gene expression nor activity was found to correlate with 5-FU sensitivity.
Further investigations to clearly define the role of ERCC1 and TS gene expression in this setting are needed.
However, point mutation of neither of these sites affected TS gene expression (Dong et al, 2000; Lee and Johnson, 2000).
To better understand the molecular mechanism of pemetrexed responsiveness, TS gene expression before and after induction therapy will be measured and correlated with the efficacy results.
When CPT-11 was combined with S-1, intratumour TS gene expression had no predictive values in terms of tumour shrinkage and survival.
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Test gene expression was normalised to 18s (dCt).
GABA-T gene expression was evaluated by isolating total RNA from liver tissues (20 30 mg) using the SV Total RNA Isolation System (Promega, WI, USA).
Treatment of BM-MSCs with TGF-β and GDF5 caused no change in KRT8, KRT19, or CAXII and a down regulation of KRT18, FOXF1, and T gene expression compared with controls.
Data sets for validation are very limited; however, in the context of a European Union-coordinated consortium, GENRISK-T, gene expression analyses on exposed and non-exposed samples were also carried out in the laboratory of B Jarzab (Poland).
The duplicated gene expression results in combination with the genome analysis suggest the possibility of long-range regulation of H2-T gene expression and/or important, but as yet unidentified nucleotide changes in the promoter or enhancer regions of the genes.
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