It also coincides with the Khatri-Rao space time (KRST) coding model of [13].
The entire 1.8 kb Pfdhfr-ts coding sequence was amplified by PCR in a 50 µL reaction volume containing 2 µL DNA, 10 mM Tris-HCl pH 9.0 at 25°C, 50 mM KCl, 0.1% Triton X-100, 2.5 mM MgCl2, 200 µM each dNTP, 0.6 µM each DhfrPfQs and DhfrPfCoasQ primers (see Table S1), with 2.5 U Taq Polymerase (Promega).
Let c be a length (KN t + N r )T coded sequence generated by underlying FEC coding scheme using d as its input.
Time after launch of generic perindopril is a continuous variable counting the number of months after the launch of generic perindopril at time t, coded 0 before the intervention and (time-18) after the intervention.
Time after launch of generic losartan is a continuous variable counting the number of months after the launch of generic losartan at time t, coded 0 before the intervention and (time-51) after the intervention.
Time after BCBV intervention is a continuous variable counting the number of months after the BCBV intervention at time t, coded 0 before the intervention and (time-36) after the intervention.
This identified three previously reported single nucleotide polymorphisms [SNP] (51I, 59R and 108N) and four novel SNPs within the Pfdhfr-ts coding region [59Y, and two synonymous L40L and one G241G].
There was no significant linkage disequilibrium between Pfcrt and the Pfdhfr-ts coding sequence and/or flanking microsatellites (Fisher's exact test, each p-value >0.00119, i.e. the adjusted p-value for 5% nominal level).
The CreER T coding sequence (Feil et al., 1996) was obtained from EcoRI digestion of pCreERt plasmid (kindly provided by Laurie Jackson-Grusbluntedunted and ligated to SP73 backbone.
In addition, a further heterozygous missense change in DSP (c.5498 A > T coding for E1833V) was identified in the index patient (35.1).
