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By transforming single cells of pTRY GUS lines with p35S: GL1, p35S: GL3 and p35S: TTG1 in the presence or absence of p35S: TRY or p35S: CPC we demonstrate that TRY and CPC can suppress the TRY expression without the transcriptional down regulation of the activators.
How is the basal TRY expression regulated by the pTRY-B fragment?
Transient TRY expression analysis was carried out by using the particle bombardment method in Arabidopsis cotyledons [ 47].
These data suggest, that the pTRY-B fragment specifically up-regulates the basal TRY expression in the context of the pTRY-A3 fragment.
Recently, it has been found that microRNA156 (MIR156 -targeted SQUAMIR156 -targetedNDING PROTEIN LIKE (SQUAMOSAan activate TCL1 and TRY exPROMOTER through BINDING to their PROTEINrs [ 21].
The repression of the activity of the three activators by TRY or CPC provides evidence that TRY and CPC represses the TRY expression directly rather than through a transcriptional feed back loop involving the activator genes.
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We first tried to examine expression of the transgene using embryonic male gonads derived from wild-type female mice crossed with four transgenic males (lines #1 to #4).
Ideally, the authors should try baculovirus expression to have a well-behaved protein that can be used in both sets of experiments.
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CEO of Professional Science Editing for Scientists @ prosciediting.com