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A technical point is the issue of how to score a truncated prediction list, where fewer than the total number of possible edges are submitted.
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Differences in the N-terminal part of the predicted protein (N-terminal truncated predicted peptides) are also not considered as prediction of start codons still remains to be confirmed by RNA sequencing.
typhimurium aptamer 5′-CCAAAGGCTACGCGTTAACGTGGTGTTGG −3′ randomndom sequence 5′-ATCCATGGGGCGGAGATGAGGGGGAGGAGGGCG GGTACCCGGTTGAT-3′ The sequences of specific target bacteria binding aptamers [17] were truncated after prediction of the secondary structure using web-based Vienna RNA software.
Several ESTs were 5' truncated, which restricted prediction by the SignalP server, yet had high similarity to secreted proteins on GenBank and were grouped with secreted genes.
Therefore we truncated crown component predictions for approach 3 in the middle of the species NWOR DBH range (as given in [12]) and applied predicted values at those points to all larger DBHs.
Therefore we truncated crown component predictions for approach 3 in the middle of the species NWOR DBH range (as given in [ 12]) and applied predicted values at those points to all larger DBHs.
This suggests that incomplete gene or transcript assemblies produced truncated protein predictions were more likely to result in BLAST alignment scores that were insufficiently strong for OrthoMCL to cluster the truncated sequences with full-length members of the same gene family.
Three WRKY genes identified as VIT_11s0052g00450, VIT_15s0021g01310 and VIT_06s0004g00230 in the 12X V1 prediction, were found to have truncated coding sequences and manual annotation was performed to correct their sequences.
This task is not routinely part of the automated genome annotation process and results can reveal that seemingly intact genes are truncated at their 5' end or interrupted and hence localization predictions can be erroneous.
ENTS predictions were then sorted by decreasing confidence and truncated to sets of the same size as the alternative classifiers.
These predictions are not always accurate, and many transcripts are truncated, so we only conclude that it is possible that some or all the diatom-derived proteins are targeted to the diatom plastid.
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