For example, sgRNA with a truncated base-pairing sequence (17 nt instead of 20 nt) enhanced the targeting specificity because truncated sgRNAs have reduced binding affinity with the target DNA and thus are more sensitive to mismatches (Fu et al., 2014).
Moreover, sequencing of leukocyte-derived amplicons demonstrated an mRNA variant with a previously described 3 base pair 5' truncated exon 2 [ 23], exon 2b (440 bp amplicon in Fig. 5) [GenBank: EU072086].
Recently, Kellmann et al. [27] have shown that the L. wollei sxtI first 912 base-pairs had 92.9% identity to sxtI from C. raciborskii T3, base-pairs 913 to 1059 and 1177 to 1189 (C. raciborksii T3 numbering) were deleted, and the 3'-end was truncated after base-pair 1227.
Among the more intriguing entries is Dorothy L. Martinez's "Between," depicting a pair of truncated figures with stockinged legs that give them a vaguely erotic cast, bending toward each other in an apparent effort to bond.
Enrollment was staggered by clinic pair and truncated at two matched sites.
Since we had already generated sequences for several jungle cat individuals using the previous primer pair, we truncated the jungle cat sequence length for the cytochrome b region.
A truncated hyperlens composed of pairs of metal-dielectric cylindrical multilayers is proposed to demagnify the diffraction limited spot to achieve deep subwavelength imaging.
For example, the gene for HELLS is frequently truncated by 75 base pairs in AML (56.7%) and ALL (37%) patients [ 111].
To avoid expression of truncated gene products, five pairs of mutagenic primers (shown in Table 4) were designed to mutate UGA into UGG.
Orphan CdiACt CdiI pairs contain truncated CdiA C-termini thought to represent remnants or reservoirs of alternative toxin domains, which can be exchanged to provide new antibacterial capability (Poole et al. 2011).
The PCR products were multiplexed and diluted for cluster generation using the cBot on a single read v.3 flowcell for the HiSeq2000 (Illumina, San Diego, CA, USA); a few samples were run on the MiSeq (Illumina, San Diego, CA, USA), with the sequences truncated to 101 base pairs to match that of the HiSeq output.
