(B ) PCR analysis of gDNA from WT and ∆PBGD clones using the primers indicated in (A ) to selectively amplify the 1.3 kb WT gene or the 960 bp truncated gene.
(B ) PCR analysis of gDNA from WT and ∆CPO clones using the primers indicated in (A ) to selectively amplify the 1.6 kb WT gene or the 1.1 kb truncated gene.
Sequences lacking all three essential histidine residues in both domains [ 16] were eliminated, as were truncated gene models (shorter than 1200 bp), or models with premature stop codons or other annotation discrepancies.
Even for the O. sativa genome, we observed numerous misannotated sequences rejected by our filtering system such as transposon elements containing proteins or truncated gene models that greatly hamper clustering and ortholog inference.
To learn the causes for gene segment truncation, we analyzed the flanking sequences for the four truncated gene segments, 4-28.1P, 4-31.1P, 4-33.1P 4-33.1P3.1P, in Region II using the repeat masking program [ 27] at the website http://www.girinst.org/censor/index.php of the Genetic Information Research Institute.
We have previously reported that overexpression of truncated gene fragments derived from the rice genes OsO3L2 and OsO3L3 could reduce Cd accumulation in transgenic rice.
Recently, we have described in T. rangeli a truncated gene copy belonging to the group II of the trans-sialidase superfamily (TrGP).
The co-expression system of the truncated gene encoding the soluble form of Kex2p (sKex2p) lacking C-terminal 201 amino acids containing the transmembrane domain (TMD) was constructed.
The expression analysis of the various N- or C-terminal truncated gene products indicated that AIO6BS probably acts as an export signal to direct its self-secretion across the cell membrane.
To facilitate expression a Met start codon was added to the truncated gene.
Furthermore, only three exclusively pRF genes occur in TG and one truncated gene is found in R. akari.
