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It is well known that rd-continuous functions (more truly regulated functions) are bounded on compact subsets of time scales.
"There are a lot of areas where federal law makes it difficult for states like Washington to move forward and understand what a truly regulated environment would look like," she said.
Multiple testing correction methods were not applied since they depend heavily on the assumption that each cDNA expression is independent and can often be too restrictive as a result of their effort to prevent all type-I errors, thus filtering out truly regulated spots [78].
To identify the most probable miRNA targets, we first adopted a classical approach, to enrich a large set of predicted miRNA targets in truly regulated genes, by identifying significant negative correlations between miRNAs and predicted target expression profiles [ 19, 20].
So far no significant evidence has been found to answer the controversial question whether this co-expression reflects a truly regulated expression or rather is a result from a persistent "leakage" from the initial high expression during early myogenesis.
We consider analytical precision one of the most critical factors in quantitative proteomics, as it controls both the number of outliers, i.e., the false discovery rate (FDR) with regard to quantification, and the statistical power of a study, i.e., the chance of a study to detect truly regulated proteins.
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We estimated that the 1.5 ratio cut-off eliminated at least two-thirds of truly differentially regulated proteins in 5 of the 6 experiments (Table S2).
If most miRNAs are truly down-regulated in prostate cancer, the global down-regulation of miRNA targets may not be causatively linked to the expression levels of the miRNAs themselves.
There were too few truly down-regulated unigenes available (see below) for GO analysis of down regulated transcripts.
High-level of co-expression is critical for the inference of co-regulation [ 8], therefore, the ICE algorithm is more likely to identify truly co-regulated gene sets that are reproducible in independent data sets.
Our 6 reference proteins were additionally chosen from different functional pathways to avoid selecting truly co-regulated proteins.
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