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Unlike a soccer ball, a bagel is not a true sphere.
To achieve this, SUVmax was measured in each hot sphere in the phantom for the OSEM images using a 3-D volume of interest, equal in diameter to each true sphere size and centred on the sphere.
The threshold level which gave the most accurate estimation of volume (compared to the true sphere volume) was determined for 111In, and 68Ga for all spheres and background activity levels.
In a further study, Knäusl et al. observed lower relative thresholds delineating the true sphere volume for PSF compared to OSEM which is in accordance with the observed MTV underestimation in the present study.
Considering that cell aggregation might confuse true sphere formation in liquid culture, we further assessed sphere formation in methylcellulose-containing semisolid culture to rule out this confusion.
A value of α = 0.3 was obtained by minimizing the average absolute error between the true sphere volumes and the volumes measured using Tbgd where the average error was calculated over all spheres and contrasts.
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The x-axes are the true sphere-to-background ratio from 1 1 to 15:15
The non-monotonic relation between RC and sphere size was independent of the true sphere-to-background ratio.
The shorter half-life of the 11C background generated an increasing true sphere-to-background ratio as function of time.
The RCs and the true sphere-to-background ratio were calculated frame-by-frame in the dynamic series.
For the PSF images (Fig. 2b), the RCs were slightly increasing for the 6 10 mm spheres as the true sphere-to-background ratios increased, whereas the RCs for the smallest spheres (3 4 mm) decreased as the true sphere-to-background ratios increased.
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