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We stress again that these frequencies should not be regarded as the true editing efficiency, but rather as a relative measure.
The patterns were then applied to predict novel RNA editing sites in T. lepidozioides transcripts; ∼60% of these predicted sites are true editing sites.
In addition, true editing sites with low expression or low editing ratios could have been missed due to stringent cutoffs in the computational flow.
For instance, true editing sites, such as A-to-I sites in pre-mRNAs of GluR-B, were not addressed in our study.
While a few non A-to-Gs (noncanonical editing) sites might be expected, none could be validated as true editing sites.
Furthermore, ~ 83% mismatches in Alu elements were A-to-G/T-to-C conversions after PSS cutoff, indicating PSS could greatly improve the identification of true editing sites.
Similar(48)
"The Impossible," the second feature from the Spanish director Juan Antonio Bayona, uses digital imagery, meticulous sound design and tried-and-true editing techniques to recreate both the violence of unleashed waters and the desolation that followed their assault on southern Thailand.
The main reason for this was the intensely negative reactions at a test screening; it didn't help that Welles was off in South America filming "It's All True" while editing was being completed.
Mr. Moore is more of a propagandist, and while most of what he portrays is technically true his editing and narrative are in large part misleading.
A major challenge in identifying RDD sites is to distinguish between the true RNA editing sites and the false positives.
The large number of predicted sites may contain sites that can be verified as true RNA editing sites, of which the top seven are listed in Table 3.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com