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As the luciferase gene is located in the 3'-end of the genome and under the control of a viral TRS, it can only be expressed from subgenomic RNAs but not from the viral genome RNA.
For imperfect TRs it is additionally necessary to restrict or penalize their imperfection, e.g. with a mismatch and gap penalty.
By fostering collaborative science in TRS, it becomes possible to speed advances in that science by fostering communication between scientists that can avoiding un-needed duplication and impact decision-making on new science that can benefit regulatory decision-making.
Our observations suggest that although polymerase identity does affect the absolute frequency of slippage errors within TRs, it is not sufficient to explain the higher mutation frequency of small dinucleotide TRs in human genomes.
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