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Additional file 3: Troubleshooting table.
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Here, we described a complete instruction of the improved capture protocol with a troubleshooting guide (Table 3) that should facilitate the preparation of enriched genomic libraries given access to either Agilent or Nimblegen hybridization equipment and any of the next generation sequencers and be applicable to other genomes.
On this basis, the significance of each design on the overall digital performance of programmable logic gates in engineered cells was studied, leading to the compilation of a comprehensive set of guidelines for troubleshooting synthetic genetic circuits (Table 1).
Worked examples, troubleshooting tips, and numerous tables and charts are provided for easy access to the data.
Troubleshooting advice can be found in Table 1.
Troubleshooting advice can be found in Table 3. Shan Lu, Yong Cao, Sheng-Bo Fan, Zhen-Lin Chen, Run-Qian Fang, Si-Min He, and Meng-Qiu Dong declare that they have no conflict of interest.
Troubleshooting advice can be found in Table 5.
Typical problems and troubleshooting in QTAP experiments are listed in Table 8.
Additional file 5: Table S1 describes other experimental pitfalls and troubleshooting solutions not considered in the main text.
Due to its popularity and troubleshooting capabilities, the EVM has become a mandatory part of a few communication standards, e.g. [[11], Tables 165, 172].
Troubleshooting skills.
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