Sentence examples for tris buffered from inspiring English sources
Wells were washed once with 300 µl Tris buffered saline (20 mM Tris and 150 mM NaCl) containing 0.1% Tris-Buffered Saline Tween-20 using a microplate washer.
After blocking with 5% non-fat milk in Tris buffered saline with Tween 20 (TBST), the membranes were incubated for 2 h or overnight with the primary antibodies.
The 96-well plates (MulitScreen Filter Plates, HTS) were washed with 250 μL 10 mM TRIS buffered saline (pH 7.3).
Between steps, the slides were rinsed for 10 min in Tris buffered saline with 0.1% Triton-X-100 (TBST).
For polyclonal antibodies, Tris buffered saline was used as negative control.
Blocking and dilution of antibodies were performed in 5% non-fat milk in Tris-Buffered Saline and TWEENTM20.
The membranes were blocked with 5% skim milk in Tris-buffered saline containing Tween-20 at room temperature (RT).
After Ponceau S staining, membranes were blocked in 2% non-fat milk in Tris-buffered saline with Tween-20® for 1 hour at room temperature.
After Ponceau S staining, membranes were blocked in 5% non-fat milk in Tris-buffered saline with Tween-20® for 1 hour at room temperature.
Blocked membranes were incubated sequentially with primary and secondary antibodies (Supplementary Table S1) and washed with Tris-Buffered Saline with 0.1% Tween 20 (TBST).
