Using the density of each food item, taken from the 2005 Canadian Nutrient File (CNF) [ 10] or manually measured (weighed by volume in triplicate), the tablespoon data for each food was converted into grams consumed per day.
For β-defensin 2 (BD2) assessment, the supernatant was analyzed using a BD2 enzyme-linked immunosorbent assay kit in duplicate or triplicate as volume allowed (Phoenix Pharmaceuticals, Burlingame, CA, USA).
"Triplicate," Bob Dylan's three-volume continuation of his exploration of American pop standards, has received widespread praise but is nominated only in the pop vocal album category; not so long ago it might have landed an overall album-of-the-year nod.
Mouse sera were diluted with 3% BSA in PBS-T as indicated in the results section and applied in triplicate using a volume of 100 μL/well on the coated plates for 1.5 hours at 25°C.
Each concentration of Qtracker705 was measured in triplicate and the total volume was 100 µL in each well after addition of [18F]FDG.
Reactions were performed in triplicate in a volume of 25 μl with the Maxima Probe qPCR master mix (Fermentas) on a LightCycler® 480 System (Roche).
Real-time PCR analyses were performed on the Prism 7500 real-time PCR system (Applied Biosystems, Foster City, CA, USA) in triplicate (final reaction volume, 25 µl) in 96-well Micro Optical plates (Applied Biosystems).
Real Time PCR reactions were carried out in triplicate in a volume of 25 μl containing 50 ng of cDNA template,1 × SYBR Green Mix Toyobo, Osaka, Japan), and 200 nM or 400 nM of forward and reverse primers.
Briefly, 1 × 104 U251 U251 and C6 cells were seeded into 96-well plates in triplicate in a volume of 100 ul per well, then incubated at 37°C for 24 h for cells adherence.
Primer efficiency reactions were performed in triplicate in volumes of 25 μL using SuperArray SYBRGreen reaction mix (SuperArray Bioscience Corp., MD).
To measure the telomere length, triplicate 25 μl-volume PCR reactions included 12.5 μl SYBR Green PCR Master Mix (TAKARA), 400 nM forward and reverse primers, 20 ng genomic DNA, and enough double-distilled H2O.
