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Validation experiment was carried out in triplicate test to confirm the reliability of the model equation.
Percent inhibition was calculated from the mean parasitemias of triplicate test and control wells as 100× (control – test)/control.
Triplicate test solutions of consisted of THF/water (1∶3.34, v/v; 80 ml) containing 2 µM, 4 µM, 8 µM and 16 µM of purified recombinant LpAFP-GFP, Type III AFP-GFP and GFP.
Triplicate test tubes including the blank were prepared.
NA, not applicable (second triplicate test run only on samples which were negative in first singlicate test).
The alkaline comet assay was performed as per the manufacturers' instructions using triplicate test samples (Trevigen Inc., Gaithersburg, MD, USA).
Similar(53)
A total of 540 tests were performed, with triplicate tests at five nominal dust concentrations and six locations.
For both sorption and desorption isotherm experiments, triplicate tests were conducted using the standard batch equilibration method.
The IC50 values were derived from the mean OD values of the triplicate tests versus drug concentration curves and expressed as mean ± standard deviation.
The extractor solvent volume (8 10 mL), vortexing time (1 5 min), centrifugation time (5 15 min) and freezing time (4 12 h) were the variables considered and were tested at three levels, including triplicate tests at the central point.
From triplicate tests performed for cell proliferation and viability, the average MTT values for tissue culture polystyrene (TCPS), unanodized and anodized titanium surfaces were equal to 0.49, 0.16 and 0.72, respectively.
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