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The errors bars represent standard deviations for triplicate batch cultures.
In this study we screened a range of these ingredients in triplicate batch culture fermentations with known prebiotics as controls.
Triplicate batch systems were set up for each treatment and each of three time points (60 minutes, 24 hrs, and 7 days), for a total of 9 bottles per treatment, to allow sacrifice and collection of all of the newly precipitated solids for analysis at the designated times.
Between 100 240 genomic copies of sxtA4 in A. catenella were found in triplicate batch cultures of ACSH02 collected at three time points with different growth rates, based on the qPCR assay (Fig. 4b).
Transgenic G. sulfurreducens strains were grown in NBAF medium [ 39] and RNA was isolated from actively growing triplicate batch cultures at an OD600 of 0.20 to 0.31.
Experiments were conducted in triplicate batch cultures using 70 × 500 mm glass tubes containing 1.2 L medium submersed in a water bath to control temperature at 24 °C ± 1 °C.
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For each soil type triplicate batches were set up with and without 1 g/L sodium azide (Sigma Aldrich).
Blank controls were run in parallel and consisted of triplicate batches containing PE samplers and water with and without sodium azide.
Microsphere diameter was measured as described in Section 2.4, using triplicate batches of non-treated microspheres and microspheres treated with EtOH NaOH for 2, 3.5 and 5 min.
Reproducibility studies were also performed, wherein 4 DNA samples were run in triplicate per batch, across 3 different batches.
A total of fourteen batches of nineteen samples were run, with at least one sample run in triplicate per batch, and one sample repeated between batches.
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