Your English writing platform
Discover LudwigSimilar(60)
We did notice that sperm spilled (by dissection) from the triple knockout testes was less abundant than sibling controls.
Figure 6 shows a representative selection of heterozygous and homozygous triple knockout testes dissected after 3 to 5 days (A F), and 10 to 16 days (G L).
Fluorescence for Don Juan is relatively unchanged throughout the length of the testes in both triple knockout homozygotes and heterozygotes, but it is consistently brighter in the seminal vesicles of the triple knockout heterozygotes, indicating less sperm in the seminal vesicles of homozygous triple knockout males (see also Figure S10).
Figure 7, A and B confirms that the elongated spermatids in triple knockout males accumulate in the base of the testes, and they appear disordered or improperly coiled.
GC proliferation in fetal and 0.5-week-old marmoset testes was quantified using sections subjected to triple immunofluorescence for OCT4/VASA/KI67, which enabled identification of proliferating and non-proliferating cells within both OCT4+ and VASA+ GC subpopulations.
Dissected testes from these double mutants strongly resemble the phenotype of the triple knockout males reported here, which suggests that the Rac1 GTPase might well be a target of a type I prenyl protease.
Lamin Dm0 localization is unaffected in a triple knockout genetic background, as visualized by immunofluorescence in situ staining of salivary glands and testes with anti-Lamin Dm0.
To better visualize the location of the mature spermatids in the testes and storage structures we constructed stocks that expressed a Don Juan GFP fusion protein in a triple knockout background.
Triple knockout males can make functional sperm but most of it accumulates at the base of the testes; also the sperm tails show signs of a surface abnormality along the length of the axoneme.
Bull testes.
Others had multiple testes that were deformed.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com