When cultured flasks became near confluent, cells were detached by mild trypsinization (TripLe Select, Gibco, Invitrogen, Paisley, Scotland) for 5 min at 37°C and reseeded onto new plates at one-third density for continued passage.
In some experiment, the viability and the numbers of tumor cells were determined using microscope counts in the presence of a trypan blue dye using tumor cells harvested after treatment with TripLE Select solution (Invitrogen) on day 3 of culture.
Each button serves double or triple purposes, selected apparently at random.
These averages are measures of the stability of the base pairings formed by the selected triple in the probe sequence with the corresponding base triple in the target sequence.
Resulting clones were transformed with a third plasmid encoding the protein to be studied, and triple transformants were selected on SC-URA-LEU-HIS.
These, in total four options (for example (CGT m, m TGC), (GCA m, m ACG)) are averaged to provide the mean effect of the flanking mismatch adjacent to 'y' and 'yc' on the selected triple.
The number of freeze/thaw cycles differed among studies, and double or triple cycles were selected.
Furthermore, we selected triple negative, basal-like FMAs to screen for BRCA mutations similar to those described in human TNBC.
Again, triple biomarkers were selected based on the best performance in SVM and significantly low P values.
After mating and selection, the resulting haploid double mutant libraries containing individual gene deletions with either mst2∆ or epe1∆ were mated again, and haploid triple mutants were selected on YES medium supplemented with antibiotics to measure cell growth.
The nature of the shift is clearly explained in (Holland 1970): modularity is in effect a weakening of the distributive laws (limiting their validity to certain selected triples of lattice elements), and orthomodularity is a weakening of modularity (limiting the validity of the distributive laws to an even smaller set of triples of lattice elements).
