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PCR was performed with the thermocycler "Master Cycler Gradient" (Eppendorf, Hambourg, Germany) using GoTaq DNA polymerase (Promega, Madison, WI, USA) or "Triple Master Mix" (Eppendorf).
The sAC gene region coding for sAC-HD (soluble adenylate cyclase haem domain) was amplified from 5 μl of different mouse tissue cDNA samples (Clontech) using triple master polymerase (Eppendorf).
The reaction mixture (20 µL) for PCR amplification of IFS2_12 contained 1× high fidelity PCR buffer, 2·5 U of Triple Master polymerase (Eppendorf, Hamburg, Germany), 0·2 m m of each dNTP, 0·4 µ m of each primer (Supplementary Data Table S1) and 10 ng of DNA template.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com