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He said any budget cuts should first look to trimming programs "where costs have spun out of control".
And there are numerous stories of nonprofit organizations trimming programs, reducing staff, or even shutting their doors as a result.
Negotiations last year indicated that Democrats are open to cutting social programs that liberals have long considered sacrosanct, as well as trimming programs that aid the poor and elderly.
As we observed different error rates caused by the vector trimming programs tested above, we were curious to see how much overall contamination remained in the dbEST [ 27].
The chromatographic data of all test ESTs was cleaned with the program phred prior to processing by one of the trimming programs (see methods).
Moreover, 174 out of the 186 incompletely trimmed pCMV-SPORT6.1-harbored-ESTs sampled from dbEST (Additional file 3) were derived from the same cDNA library which had been used to study the performance of the three trimming programs in Table 4. aNumber of ESTs which were cloned into the indicated vector remained to be contaminated with vector.
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Faced with the fragmentation of audiences and a decline in advertising revenue, broadcasters are trimming programming budgets, and unscripted shows are generally less expensive than fictional dramas, movies or other fare.
Prior to assembly, the reads were re-trimmed by the Figaro vector trimming program [10].
On the other hand, if vector does get overlooked by the trimming program, the short sequences will remain untrimmed.
Based on the observations above, a hypothesis was made that the trimming program neglected the circular form of the vector DNA which resulted in erroneous trimming of some of the EST sequences.
The trimming performance of the three programs against CVS or RVS vectors were all compared in terms of number of incompletely-trimmed ESTs, number of low quality (e.g. shorter than 100 bases) ESTs discarded by a trimming program, and the average lengths of trimmed ESTs.
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