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The quality of paired-end data was assessed using FASTQ and subsequent quality trimming performed with the Trimmomatic software [ 52].
Trim and fill analysis showed that the outcomes were not changed without trimming performed, and the outcomes still showed statistical significance when a fixed effect model was used.
Simulation analyses show that the character trimming performed by BMGE produces datasets leading to accurate trees, especially with alignments including distantly-related sequences.
However, new samples taken at pasture in June were negative, and at claw trimming performed by a professional trimmer at the end of the grazing season the cows were reported to have healthy claws.
After the stationary-based trimming performed by BMGE, all Saccharomyces sequences induce pairwise Stuart's test p-values > 0.85, the lowest p-values being induced by the outgroup species (i.e. varying from 0.1000 to 0.3675 for the sequence pairs C. albicans - S. kluyveri and C. albicans - S. castellii, respectively).
Similar(55)
Vector trimming was performed manually and contig assembly was performed using the DNAStar software (low stringency settings, no vector trimming).
As a last step, a trimming is performed to remove all ligand copies situated more than 3.5 Å from the target.
This increased vertical development of the mandibular molars was expected since acrylic trimming is performed with this objective, based on Harvold's principle of differential eruption, depending on the need to correct the overbite that normally accompanies class II, division 1 malocclusion [13],[13].
The wetland setting indicates that the track components must have arrived prefabricated, before being assembled on site, although the presence of wood chips and chopped branches indicates that some trimming was performed locally.
Sequencing was conducted with the BigDye kit from ABI (Foster City, USA) using standard forward and reverse primers; pre-assembly trimming was performed with a modified version of Phred [56], [57].
Read trimming was performed using Trimmomatic [ 56].
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Justyna Jupowicz-Kozak
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