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In this study, the observed trend of lower CelS protein expression under fast growing conditions on Z-Trim and cellobiose (data not shown) is consistent with this type of regulation.
In a test with PartiGene using the same nine sets of EST data as the other programs, almost ten times as many ESTs (2014) were trimmed into <100 bases long segments (data not shown) and were finally removed, in comparison with the results shown in Table 3.
Additional primer extension analysis with a specific probe within PAB0571 suggests that a unique RNA precursor is transcribed from this locus and subsequent processing events including 3' end trimming generate the different sRkB RNA species (data not shown).
Initially, we unsuccessfully looked for candidate retrotransposons in E. multilocularis by searching for a short distance (<20 kb) between BLASTN searches for ta-TRIMs and TBLASTN searches for RTs (data not shown).
After trimming and mapping, approximately 96% of the reads were mapped to the genomes (data not shown) indicating that RNA-Seq provides high quality reads suitable for Xanthomonas transcriptomics.
Whisker trimming did not result in an increased incidence of immature slow synapses at P8 P9 (data not shown), suggesting that the time period in which such transient contacts are formed is predetermined and this acts as the substrate for experience-dependent plasticity.
In particular, although lacking TSDs, it seems that the EgRep element previously described for E. granulosus by Marin et al. (1993) consists of a solo-LTR from a ta-TRIM element, embedded within a larger, sparsely repeated sequence lacking any other distinctive elements (data not shown).
A similar percentage in matching reads was also obtained when sequences were trimmed for low-quality bases before mapping or when the whole genomic sequence of C. intestinalis A was used as a reference (data not shown).
All clones showing pathological SNPs were excluded (data not shown).
(detailed data not shown).
(Data not shown).
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