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Blood samples (7 mL) were obtained during the baseline interview and/or at each trimester visit.
Furthermore, if a woman missed her trimester visit then this was treated as missing data and no estimation was made.
Blood (7 mL) was taken from each of the participants during the baseline interview and/or at each trimester visit.
Body mass index (BMI) was computed as kg/m based on weight and height measured by a trained research assistant during a second trimester visit.
In the first pregnancy trimester visit, we measured maternal height, and the mothers reported their prepregnancy weight and paternal weight and height.
- Request for ioduria during the first trimester: the midwife will request an ioduria test and will explain the woman how to correctly collect the urine and will program the appointment for the second trimester visit of the pregnancy.
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The schedule of home visits in the study included preconception visits, first trimester visits, and third trimester visits.
Logbooks of food intake and physical activity were collected at enrollment, during the first and third trimester visits and one year after delivery.
If we consider that childbirth preparation might be included in the last trimester counts, the frequency of third trimester visits is quite low (eight sessions of childbirth preparation per woman are fully reimbursed by social security– see Additional file 1 for details).
During the 1st-trimester visit, all women also underwent a 50-g glucose challenge test (O'Sullivan test).
Notably, although the earliest visit among all study participants was at 3.8 weeks of gestation, the average first-trimester visit occurred at 13.7 weeks.
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