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Therefore we have studied first trimester cells to investigate their potential to differentiate into either white or brown adipocytes.
During the NHP third trimester, cells in the OL lineage undergo maturational changes in which OL progenitors differentiate into promyelinating, and then myelinating OLs.
This difference might reflect the greater proportion of cells undergoing adipogenesis (>30%) in our study, although a fundamental difference between the first trimester MSCs used here and second trimester cells cannot be ruled out.
Since the effects of T3 were more prominent in first trimester cells and particularly so in first trimester macrophages, we investigated this using conditioned media from first trimester total decidual cells and first trimester decidual macrophages.
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Lastly, we are using an SV40-transformed first trimester cell line rather than primary cells or choriocarcinoma cancer cells, which, to our knowledge, is one of the best available models for studies such as ours.
Preliminary studies have failed to find an association between first-trimester cell-free DNA levels and preterm birth, whereas there is conflicting evidence as to whether elevated second-trimester cell-free DNA is associated with a subsequent spontaneous preterm birth clinical event.
Furthermore, miR-146a was significantly downregulated following exposure to both nicotine and benzopyrene in TCL-1 (immortalized human trophoblast cell line), a cell line derived from third trimester extravillous cells, whereas no impact due to either agent was observed on the remaining miRNAs.
In this issue, Apps et al. 9 demonstrate that significant amounts of the β2m-associated HLA-G dimer are expressed at the cell surface of normal first trimester trophoblast cells.
Our own recently published data, however, demonstrate that the miRNA profiles of these two cell lines are quite differing, surprisingly with JEG3 encompassing an miRNA profile that is closer to primary first trimester trophoblast cells than that of the HTR8/SVneo cell lines [ 11].
This study aimed to investigate the expression and function of NOD1 in first trimester trophoblast cells, and evaluate the potential role of trophoblast cells in infection-associated inflammation.
Overall, we cannot find evidence for protein expression of ligands for the NK cell receptors NKG2D and 2B4 on normal first trimester trophoblast cells.
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