These were necessary as the sheets were trimmed to remove any previous wear and tear, which was assumed to have an effect on the experiment.
Sequences of the respective clone libraries were trimmed to remove vector and primer sequences using MEGA6.0 software (Tamura et al. 2013).
The sequences were trimmed to remove primers and barcodes, quality filtered using sickle v1.33 with a minimum quality score of 20, assembled in mothur and aligned to SILVA 123 database.
The estimates are trimmed to remove industry/year observations in which the calculations from the CPS implied an absolute annual change in total person-hours of at least 50percentt.
The remaining sequences were trimmed to remove the flanking 18S ribosomal RNA (rRNA) and 28S rRNA sequences so that sequences started at 5′-GAGA and finished at TTTATTGNN-3′.
All reads were trimmed to remove primer sequences and low-quality sequences.
Sequence reads were automatically trimmed to remove poorly resolved base pairs as determined by quality scores.
UGT1A1 and UGT1A6 sequences were aligned by Clustal X, adjusted manually, and trimmed to remove overhangs.
The Illumina 1G sequencing reads were first trimmed to remove the adaptor sequence.
Sequences were trimmed to remove overhangs and aligned using ClustalW [54] without gap penalties to preserve structural alignments.
Illumina sequences were trimmed to remove primer and transposon sequences and mapped to the zebrafish genome assembly v8 using BLAT.
