Sentence examples for trim length from inspiring English sources

The trim length with the highest percentage of mapping was selected as the trim length for all reads from each library.

The final trim length (50 bp) used in the assembly was chosen after trimming to different lengths (40 80 bp) followed by quality filtering and then the assembly with highest n50 and fewest number of contigs was selected as the "best" assembly.

Next, mapping rates were compared between the different trim lengths.

Reads with a post-trim length of less than the k-mer length (20 nucleotides) were also discarded.

Surviving reads were trimmed again, this time at the first instance of a high-abundance (>50×) k-mer; again, reads whose post-trim length was less than 20 nucleotides were discarded.

Reads were filtered by dynamic end trimming with a Phred score of 30 as minimum quality and a minimum trimmed length of 50 bp.

To determine the trimming length, 50,000 reads were randomly selected from the sequence data set, then trimmed to varying lengths and aligned to the reference genome (Stajich et al. 2010).

In brief, all reads were trimmed (length 22 bp) and aligned against the current version of the human genome (GRCh37), using the publicly available software Bowtie [ 19].

These sequences had an average trimmed length of 519 base pairs and were assembled into 348 clusters and 1332 singlets, resulting in the final annotation of 1686 unigenes.

The other default parameters used in our pipeline were as follows: read trimming length = 5; SNP detection threshold = 20; number of shared SNPs = 1.

The CB4856 data was based on longer Sanger reads (average trimmed length was 130 bp) while the CB4858 data was based on short read sequencing.