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Cassandra is a particularly interesting group of TRIM elements.
Still, there is little information on TRIM elements in maize so far.
Seven TRIM elements and three Solo-LTRs related to elements belonging to the family Copia-1_CH were also identified.
Third, many angiosperm species bear retroelement signatures in regions flanking the 5S insertions resembling Cassandra TRIM elements (Kalendar et al., 2008).
It appears that TRIM elements have expanded in size over a relative long period of time in plant genomes (Kalendar et al. 2008).
ta-TRIMs are only the second family of TRIM elements discovered in animals, and are likely the result of convergent reductive evolution in different taxonomic groups.
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Moreover, Wukong elements are the first TRIM element family that possesses many members with multiple LTRs in larger genome sizes.
Methylation might be associated with the presence of a TRIM element within the promoter region, but the presence of the TRIM element alone cannot explain the phenotypic variability observed in 'Honeycrisp'.
These same thermocycling conditions were used to study whether the TRIM element is transcribed in the cultivars studied.
Results confirmed the presence of the TRIM element in each of these cultivars in a position identical to that observed in 'Royal Gala'.
We checked for the presence of a TRIM element upstream of the MYB10 locus in 'Honeycrisp' (blushed and striped), 'Connell Red', 'Fireside'1807807' (green selection) and 'Geneva' (ultra red cultivar) via PCR, combining a primer designed from the TRIM element (TRIM forward primer) with one designed from the promoter region of MYB10 (primer -1873).
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