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Fig. 8 The RS trigger sequence diagram.
A single-stranded DNA (ssDNA) probe with two uracil bases and a trigger sequence were designed.
Firstly, bleomycin cleaved bicyclo-hairpin probe to release a trigger sequence.
Then the trigger sequence initiated strand displacement amplification reaction to produce numerous G-quadruplex forming sequences.
Subsequently, the trigger sequence was annealed with the reporter probe, initiating the polymerization and nicking amplification reaction.
Although the substitutions did not bring the overall sequence in closer agreement to the consensus trigger sequence, they increased coiled-coil folding and stability.
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In the presence of live S. typhimurium, single-stranded trigger sequences were released from aptamer-trigger sequences complex, initiating a branch migration to open the hairpin template I containing complementary scaffolds of AgNCs.
Finally, the trigger sequences hybridize with padlock DNA to initiate circular rolling circle amplification and generate enhanced fluorescence responses.
Then, the AP in YJS can further act as primer to initiate strand displacement amplification, releasing numerous trigger sequences.
The versatile Spinach.ST sensor can detect DNA or RNA trigger sequences and can readily distinguish single-nucleotide mismatches in the trigger toehold.
Although specific trigger sequences have not been clearly identified in dimeric coiled coils, higher-order coiled coils, as well as other oligomeric multi-protein complexes, may require such sequences to nucleate and direct their assembly.
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