The acute inflammatory response involves the release of reactive oxygen and nitrogen species that were shown to trigger mutations and intra-chromosomal recombination in vitro10.
In Deadpool, Wade gains superpowers after being injected with a serum that "activates any mutant genes" lurking in his DNA when combined with extreme stress to "trigger mutations".
These results strongly support the view that, even in non dividing cells, mismatch repair can trigger mutations at distance from the initial mismatch.
In order to examine if AID is able to trigger mutations in the absence of DNA replication in a non-B cell line, we developed a highly sensitive assay based on the reversion of nonsense mutations of the EGFP gene cloned in a non replicating vector.
In addition, we have observed that one sgRNA does not trigger mutations in Cas9 nickase transgenic flies, suggesting that the Cas9 nickase system significantly prevents off-target effects.
Thus, modified TFOs containing suitable chemical compounds have been used to develop artificial nucleases [ 11, 12], to induce recombination in mammalian cells [ 13], and to trigger mutations in target DNA [ 13- 16].
The reason is that there are only a few common cancer-triggering mutations, and drugs to deal with them.
Other triggering mutations are numerous, but rare so rare that no treatment is known nor, given the economics of drug discovery, is one likely to be sought.
Here radiation has left the land flat and bleak for miles, triggering mutations in flora and fauna and leaving everything ill.
Here radiation has left the land flat and bleak for miles, triggering mutations in flora and fauna and leaving everything ill" ("Fields of dreams", August 24th).
