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Here we propose a modified triangle threshold method to segment the cell body from the background.
The method first segments the cell from the background, using a modified triangle threshold method, and then extracts the filopodia using a series of morphological operations.
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The triangle thresholding method was originally proposed by [ 24] to segment sister chromatids from microscopy images.
The presence of the vertical grating (open triangles) increases thresholds for all surface slants (except for the steepest slants for observer DT), reflecting an overall decrease in visibility of the horizontal component.
Based on this assumption, a 0.5-mm black triangle represented the threshold of acceptability in the younger group, whereas in the older group, 1.5 mm was the limit of acceptability.
The graphs are estimated as local linear regression models on both sides of the eligibility threshold, using a triangle kernel and the default bandwidth from Imbens and Kalyanaraman [18].
The images were background corrected as described above, flattened using the rolling-ball method in ImageJ, and thresholded using the triangle method.
Images were thresholded using the triangle algorithm in Image J. The fractional intensity of the clustered regions was then calculated by dividing the integrated intensity of the thresholded image by that of the non-thresholded image.
Legend: Max = maximum; asterisk = maximum performance threshold for each individual; triangle = current performance level; black-white circle = initial performance level.
The probability of a link to be part of a triangle decreases with distance, until a threshold distance of 40 km, after which the probability is constant.
To control the number of artificial vertices, the new vertex x is created only if the sum of pairwise distances of the triangle among u, w and y exceeds the threshold Δ.
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