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We emphasize that the subject was allowed as much time as desired to plan the two movements on each trial; timing did not start until the subject began to move.
For single trial estimates, category decoding was equal across all trial timing conditions while item-level information and memory effects were better detected using slow trial timing.
Jittered and non-jittered versions of the quick trial timing did not differ significantly in any analysis.
Here, we tested several designs that varied in the number of trials and trial timing within fixed duration scans while participants encoded images of animals and tools.
Trial timing conditions with fixed onset-to-onset timing ranged from slow 12-s trials with two repetitions of each item to quick 6-s trials with four repetitions per item.
We assessed the effect of trial timing on three dependent measures: category-level (animals vs. tools) decoding accuracy using a multivoxel pattern analysis, item-level (e.g., cat vs. dog vs. lion) information estimates using pattern similarity analysis, and memory effects comparing pattern similarity scores across repetitions of individual items subsequently remembered vs. forgotten.
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Panel B in Figure 1 provides a schematic diagram of the recognition memory task used and trial timings.
Each predictor was a boxcar function that reflected the trial-by-trial timing of the auditory and movement epoch of the condition.
The inter-trial timing of the TMS pulses and the direction of current were controlled using Matlab (Mathworks, MA, USA) using Activewire (ActiveWire Inc., Campbell, CA, USA).
DOI: http://dx.doi.org/10.7554/eLife.01982.011 The reliability score was computed by comparing each cell's time-tuning to that of control data obtained by artificially disrupting any trial to-trial timing relationships in calcium activity.
In both trials, timing of surgery was not studied, and no cost-effectiveness or predisposing factors were evaluated.
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