Resting and exercise induced metabolic rate and scope, and sustained running speed were analysed by 3-way ANOVA with thermal acclimation and exercise as independent, fixed factors, and ambient test temperature at which trials were performed as repeated measure.
In a small pilot trial, brain temperature under endovascular cooling showed that even under normothermia (36.5°C) brain temperature almost reaches body core temperature [ 11].
However, the use of therapeutic hypothermia has been recently challenged by the results of our TTM trial (Target Temperature Management After Cardiac Arrest), which showed that lowering body temperature to 33°C in unconscious survivors of out-of-hospital cardiac arrest did not confer protection compared with 36°C [ 35].
Another trial compared temperature control strategies in a tertiary trauma ICU and randomized patients to either aggressive temperature control or a permissive strategy [ 47].
Given the negative results of the previous outdoor trial without temperature control, for this trial the control system was set up so as to allow the culture temperature to increase during daylight but never surpass 40°C.
During the trial, water temperature was 17 21 °C; pH was approximately 7.6, dissolved oxygen was above 6.0 mg/l and photoperiod controlled to 12-h light and 12-h dark.
In the trial the temperature in the antechamber was −60°C, whereas in the proper chamber it reached −120°C.
At the start of the trial, the temperature was raised from 18°C to 25 ± 1°C over the course of 1 h by adding heated water to the system.
In greenhouse trial, the temperature of greenhouse often exceeded 45°C in summer, and reached 50°C at the noon, which restrained growth of tall fescue and caused severe thermal damage.
In laboratory trials, three temperature regimes (37.5 °C for 60 min, 40 °C for 30 min and 42.5 °C for 20 min) were tested on a range of temperate taxa commonly found in sea chests.
