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One group received standard extinction of the CS in Context B, while another group received explicitly unpaired treatment in Context B where food was presented only during inter-trial intervals (and not paired with the lever).
Inter-trial intervals of 10 min were used throughout.
The inter-trial intervals lasted one second following participants' response.
Inter-trial intervals varied randomly between 1 and 2 seconds.
The Paired group received ten odor-shock pairings with 2 min inter-trial intervals.
The fixation point was now a small white dot, visible only in inter-trial intervals.
CS-US pairings were presented with 2 minute inter-trial intervals (ITIs).
To prevent carry-over effects, (pseudo-randomly jittered) inter-trial intervals of 6000, 7000, or 8000 ms were adopted.
Mice went through three trials with 45- to 60-minute inter-trial intervals on each of two consecutive days.
23 wild type and 13 Dyrk1A+/− mice were tested over 4 days (4 trials/session, 10-min inter-trial intervals).
The trials were presented in a pseudo-randomized order, with inter-trial intervals randomized between 1 and 6 s.
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