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To assure that each action was not having an effect on the following actions (e.g., whereby a subject pressing on a beautiful image for a long period of time, may press for a lesser period in a following trial), an analysis of variance was performed for the effect of preceding trial behavior (independent variable) on each subsequent trial (dependent variable) across and within subjects.
However, these perceived benefits were thought to be trial dependent.
Reward was calculated adaptively on each trial dependent on when gaze arrived at the target and was displayed numerically.
This relationship is, however, expected to be treatment and trial dependent, and will thus change from baseline to end-of-trial.
Although participants were informed that the outcome of each trial dependent on their choice, the task used a pseudo-random design with three different sequences of trials: (1) Trials after 3 consecutive winning outcomes (WIN).
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Another possible extension would be to investigate the different sample sizes of trials dependent on whether the primary endpoint of the trial is based on efficacy or feasibility.
We then used multivariable regression to examine the relation between log transformed number of randomised trials (dependent variable, transformed owing to positive skew) and log transformed total disability adjusted life years (global disability adjusted life years) and log transformed ratio of disability adjusted life years in low income regions to high income regions (independent variables).
We implemented a hierarchical model where ( {y}_{ijklm} ) follows a left-censored normal distribution ( Nleft({omega}_{ijklm},{tau}_{jBig| i}right) ) with predicted value ( {omega}_{ijklm} ) and a trial-dependent precision ( {tau}_{jBig| i} ).
Importantly, the immediate spontaneous reactivations following CS/US stimulations exhibited a clear trial-dependent property, that is, the numbers of reactivations increase in proportion to trial numbers.
Moreover, it has been shown in the trace fear conditioning paradigm that conditioned tone responses and tone-shock association patterns undergo trial-dependent increase in the numbers of replay during learning, correlating tightly with increased immediate freezing [12].
Again, the group data (from six mice) also confirmed that the trial-dependent increases in the total numbers of reactivations (Fig. 3H) were highly correlated with the averaged amounts of immediate freezing over learning trials (r = 0.9551, p<0.001).
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