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Based on qPCR verification, trends of expression differences of some above genes agreed with those obtained by Illumina sequencing (Table 2).
Results indicated that, trends of expression differences of these genes detected by qPCR agreed with those concluded by Illumina sequencing (Table 2).
The consistent trends of expression of these proteins within similar proximity strongly suggest a functional interaction in GBM.
The transcription burst followed two major trends of expression in atm: (i -loss or severe attenuati -loss delay, and (ii)-inverse and/or severestic, attenuationspecific.
The transcription burst was almost exclusively AtATM-dependent or weakly AtATR-dependent, and followed two major trends of expression in atm: (i -loss or severe attenuati -loss delay, and (ii)-inverse and/or severestic, attenuationspecific, enanding one to delaynguish IR/and pathway constii -inverse
The induction kinetics of TG (Basic), C2TG (Delay), and TC2G (Control Delay) circuits (Fig. S3 in Supporting Information S1 section B) shows similar trends of expression of the Basic and Control Delay circuits, and large overshoot with the Delay circuit at all inducer concentrations.
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Genes related to sulfur metabolism that exhibited different trends of expressions between the treatments were chosen for validation (genes and primers are listed in Table 1).
Some of these genes and their trends of expressions were found consistent in our SAGE and proteomic data.
A putative lactate permease mRNA (DVU2285) was down-expressed in biofilm cells, and a similar trend of expression was observed with exponential-phase, planktonic cells [ 29].
Basically, our method examines the trend of expression changes of target genes of a miRNA.
We considered a trend of expression change when expression level was altered more than 1.5-fold, and strong expression change if altered >2-fold[8].
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