The general trends of transcript accumulation in the shoots of Pi-deprived plants were similar to those found in the roots, except for AtPHT1 7 and AtPHT1 8, where transcript abundance remained constant and similar to the control plants.
Similar expressional trends of transcripts of wound-responsive enzyme arginase in both the infected plant varieties emphasized wounding caused due to fungal penetration.
RT-qPCR analysis of leaves expressing Arabidopsis WRI1 showed the temporal trends of transcripts selected as 'markers' for key metabolic pathways one to five days after agroinfiltration.
Noteworthy in its exception to the general trend of reduced transcript levels in response to coilin overexpression, U2 snRNA levels were not reduced upon overexpression of WT or S489D coilin in HeLa cells.
The opposite trend of increasing transcript abundance from Phase I to Phase III was observed for functional groups that included transcription (11), protein synthesis (12), protein fate (14), protein with binding function (16), and to a lesser extent with interaction with cellular environment (34).
Conversely there is a trend of MHC transcripts being decreased associated with larger polysomes, in agreement with the reduced content of myosin proteins (Supplementary Figures S11B-C).
The per-transcript estimate is considered to be more appropriate when large numbers of replicates (≥ 4) are present, while the functional form is considered to be more appropriate when small numbers of replicates (≤ 2) are present, in which case information is borrowed from the general trend of all transcripts.
Given the variations between HIF-α protein expression and HIF transcriptional activity, we next examined whether the expression of HIF-regulated proteins mirrored the trends of their transcript levels.
There was a non-significant trend of increasing cry1 transcript abundance over the sampling timeframe (Figure 6A).
The trend of changes in transcript levels observed in the primer extension assays was similar to that reported in the microarray analysis, with an exception of GSU1836 (glnB), which encodes a PII nitrogen regulatory protein.
