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A contrast between the trend of proliferation and the trend of cell elongation was observed.
The same trend of proliferation inhibition was observed for lung cancer A549 cells transfected with miRNAs 663, 744 and siRNA against eEF1A2 (Supplementary Figure 1A).
It was not possible to observe the trend of proliferation at longer times because, after 120 h, cells treated with the optimal concentration showed a confluence so high that it prevented further growth due to contact inhibition.
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The results show a trend toward increased cell proliferation in the treated samples in the pilot study (Qigong/sham CFE ratio > 1.0), a statistically significant trend of increased proliferation following Qigong treatment in the formal study, and a nonsignificant, slight increase in proliferation following Qigong treatment in the replication study.
This trend of decreased proliferation continued into later stages of culture (D7 to D10).
A Jonckheere Terpstra test was carried out using SPSS V.16.0 to analyse the trend of the proliferation and mitotic rates across the four-cell compartments.
In this study, specimens from animals over 1 year of age were used and a trend of higher proliferation rates in younger animals was present, when compared to the older animals (up to 8 years of age).
In animals over 1 year of age, a trend of higher proliferation rates in younger animals was present, when compared to the older animals (up to 8 years), but age did not influence the species and anatomical location differences or the chondrocyte reaction to growth factor treatment.
Neither DAPT nor Jagged peptide significantly affected the proliferation rates of keratinocytes or fibroblasts, although there was a trend towards inhibition of proliferation with both treatments (Fig. 5i, j).
There were trends of increasing proliferation in the order: HMPV<HRSV<HPIV3<IAV, and greater production of interferon-γ and tumor necrosis factor-α by proliferating cells in response to IAV, but differences were not significant.
Other experts argue that the incentive it creates for countries to criminalise trafficking will not do much where law enforcement is weak and the economic reasons to migrate for work are strong.Trafficking is also an example of another trend: the proliferation of indices on similar topics.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com