Pairwise FST analysis revealed variable trends of differentiation between sub-populations.
There was very little differentiation among subpopulations and a weak trend of increasing differentiation with geographic distance.
D est values were 0.226 for farm and 0.229 for wild trout and displayed the same trend of pairwise differentiation shown by FST values (Supporting information Table S3).
A trend of increased adipogenic differentiation was observed for yMSCs cultured in aSerum (1.34±0.20) compared with their counterparts in ySera (1.11±0.07, P=0.112), but did not reach statistical significance.
In summary, although we do observe supportive trends for a number of differentiation genes (Mef2c, Alx1, Alx4, and Alpl), results are mixed for other targets, which may be explained by compensation in vivo, residual receptor expression, or other factors.
Averaging GSFST over the top ten regions shows a general trend of a smaller degree of differentiation between the sub-Saharan African group and other groups.
In this research [99], mouse neural stem cells (NSCs) line C17.2 were incubated with TiO2 NPs (coated with SiO2; 80–100 nm; 50, 100, 150, 200, 250 μg/ml) for 12, 24, 36, 48, 60, 72 h, or 7 days to determine the effects of TiO2 NPs on the differentiation trend of neural stem cells.
The fraction of methylated CpGs was calculated over all sequenced alleles 10-200 per sample) and revealed a low degree of SOX11 methylation although a trend of increased methylation during differentiation was observed.
The comparison of the acinar genes from day 3 to day 5 in the control group (matrigel only) indicates a decreasing trend which suggests that additional supplementation of differentiation factors may be needed to induce progression of differentiation on matrigel.
These eight genes showed the same trend of Klf5 expression during ESC differentiation and, moreover, a different transcriptional control by Klf2, Klf4 and Klf5 with Igfb3, Niban and Perp responding only to Klf5.
