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And according to this 16S rRNA gene based neighbour-joining tree, strain GFP-2 is located in a separate clade with B. velezensis CR-502T, B. amyloliquefaciens DSM 7T and B. siamensis KCTC 13613T, indicating their closely phylogenetic relationships (Fig. 2a).
In their resulting tree, strain 1,231,408 still occupied a "hybrid" position similar to the one observed in the unfiltered tree presented here (fig. 1 A).
From our phylogenetic tree, strain R15892 has probably lost a pCS1-type plasmid, being closely related to the tcsL + strains ATCC9714 and JGS444/445.
To accomplish these aims, extensive microscopy analyses were carried out to initially characterize four different stages of MS development in a smoke tree strain of V. dahliae.
The deletion of the HOG1 gene (VDAG_08982) from the smoke tree strain resulted in significantly reduced and delayed production and developmental progress of MS in vitro (Xiao et al., unpublished data).
The level of nucleotide polymorphisms between YJSH1 and S288c (0.57%) is very similar to the level separating S288c and AWRI1631 (wine strain), YJM789 (pathogenic strain), M22 (vineyard strain) or YPS163 (oak tree strain) [ 21, 36], but, interestingly, YJSH1 was grouped closely with sake strains, consistent with their geographical distributions.
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It's like a building in Hong Kong that is a sapling, a little tree, straining for light".
Consistent with the NS5B tree, strains isolated in Zhenjiang are also dispersed together with the strains from whole China in C/E2 tree.
Such groups include sake strains from Japan, oak tree strains from North America, and strains isolated from Europe or vineyards.
The strongest candidate is strain PittEE; although different trees assigned it different close relatives, it was always most closely related to a strain of low competence (strain 22.4-21 in the MLST trees, strain 86028NP in the Genome tree, and strain R2846 in the Uptake/transformation tree).
In reconstructed phylogenetic trees strain KT71T belongs to a branch comprising the recently described species Haliea salexigens and Spongiibacter marinus as well as numerous 16S rRNA gene sequences retrieved from uncultured marine bacteria (Figure 1).
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