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(3) Tree distance calculated from the phylogenetic tree calculated by FastTree in the previous section.

The techniques we describe are based on modifications of a minimum-spanning tree calculated from a weighted, undirected graph.

An example tree calculated according to the parameters given in Table 1 is shown in Figure 1.

Probably erroneous sequences (with highly unlikely positions or extreme branch lengths, based on a neighbour-joining tree calculated with all sequences) were identified by eye and omitted.

Maximum-clade credibility tree calculated with BEAST, using the concatenated alignMaximum-clade credibility.

Gene trees were rooted using SoftParsMap (Berglund-Sonnhammer et al. 2006), and a species tree calculated as described below.

In contrast, on a tree calculated from the 16S rRNA gene sequences, no clear clustering was observed.

This compares the alignment generated using the guide tree, calculated using the embedded distances against the corresponding HOMSTRAD structural alignment.

As organismal tree we use the consensus tree calculated from the individual gene trees using the majority-rule consensus method (see Methods).

First order taxon jackknifing (Lanyon, 1985; Siddall, 1995) was used to determine the robustness of tree topology, with a consensus tree calculated from all analyses.

The remaining 3000 trees were imported into P AUP* 4.0b10 [ 54], and posterior probabilities were obtained from the 50% majority-rule consensus tree calculated from these.

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